Project Description
Utilize biotechnology to solve a current wold issue, given an unlimited budget. Our team decided to solve energy consumption, and along the way cleaner air by modifying plants to make them glow. Unlike standard lights that have huge carbon emissions attached to them, plants give of only oxygen, and is an autoluminescent system.
Key Terms and Concepts
Gene Gun: A method where gold particles are coated with the plasma, an are fired to the target region of a gene.
Heat Shocking: Putting cells in a temperature higher than the optimal temperature for the cell's function, where the cell is exposed to stress changes in temperature, infections, etc.
Electroporation: A method to allow transport agents, like bacteria and yeasts, by subjecting the cell into a conductive solution, with electrical pulses to open the porous membranes to allow transport agents.
Xylem Transport: The xylem, analogous to the human circulatory system, is a tube that transports water and solutes from the roots to the leaves.
Plasmid: A genetic structure that can replicate independently of chromosomes, that is usually the circular DNA found in the cytoplasm of prokaryotes (bacterium or protozoan)
Luciferin: The light producing substance
Luciferase: An enzyme that catalyzes the reaction, creating Luciferin.
Agrobacterium: Soil bacteria that works as a natural genetic engineer, which invades plants through wounds in the stem or root.
Enzyme: A byproduct of a living organism that catalyzes for certain biochemical reactions.
Restriciton Enzyme: An enzyme used by certain acteria that can cleave DNA molecules at specific sequences of bases. (Biochemical scissors).
Ligase: An enzyme that covalently links two ends of RNA or DNA molecules.
Bioluminescence: Biochemical emission of light by living organisms such as fireflies and deep-sea fish.
Autoluminescence: Luminescence of a substance die to energy originating within itself.
Heat Shocking: Putting cells in a temperature higher than the optimal temperature for the cell's function, where the cell is exposed to stress changes in temperature, infections, etc.
Electroporation: A method to allow transport agents, like bacteria and yeasts, by subjecting the cell into a conductive solution, with electrical pulses to open the porous membranes to allow transport agents.
Xylem Transport: The xylem, analogous to the human circulatory system, is a tube that transports water and solutes from the roots to the leaves.
Plasmid: A genetic structure that can replicate independently of chromosomes, that is usually the circular DNA found in the cytoplasm of prokaryotes (bacterium or protozoan)
Luciferin: The light producing substance
Luciferase: An enzyme that catalyzes the reaction, creating Luciferin.
Agrobacterium: Soil bacteria that works as a natural genetic engineer, which invades plants through wounds in the stem or root.
Enzyme: A byproduct of a living organism that catalyzes for certain biochemical reactions.
Restriciton Enzyme: An enzyme used by certain acteria that can cleave DNA molecules at specific sequences of bases. (Biochemical scissors).
Ligase: An enzyme that covalently links two ends of RNA or DNA molecules.
Bioluminescence: Biochemical emission of light by living organisms such as fireflies and deep-sea fish.
Autoluminescence: Luminescence of a substance die to energy originating within itself.
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Proposal
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Mentor Notes
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Reflection
Oeerall this project went well. Initially I was concerned about the number of people in the group (7), and the people in it (my friends). I was worried that with a large group job delegation would be difficult, making agreements would be difficult, and productivity would be lost to socializing. But none of that was the case. Through the project, the work was evenly divided, everyone was on task, and sometimes too caught-up in the little details about marketing. Because of our productivity, we forgot about efficiency. We would finish with the different stage of the project quickly, but never fully adhered to the guidelines or the actual task at hand, which caused us to rework some of the stages. I also would've liked to include other's voices more, as it can be difficult with a seven-member group full of people with leadership characteristics. I would have liked the time and resources to physically conduct our lab to see where we needed to improve, and how the lab would turn out, But in all, it was a fun and enjoyable project.
- Nihal Nazeem
- Nihal Nazeem